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Updated: Mar 27, 2026

Synthesis of an Intein-mediated Artificial Protein Hydrogel
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Tuning Reaction and Diffusion Mediated Degradation of Enzyme-Sensitive Hydrogels
Stacey C Skaalure1, Umut Akalp2, Franck J Vernerey3
1Department of Chemical and Biological Engineering, University of Colorado, Boulder, CO, 80309, USA.
Abstract:
Enzyme-sensitive hydrogels are promising for cell encapsulation and tissue engineering, but result in complex spatiotemporal degradation behavior that is characteristic of reaction-diffusion mechanisms. An experimental and theoretical approach is presented to identify dimensionless quantities that serve as a design tool for engineering enzyme-sensitive hydrogels with controlled degradation patterns by tuning the initial hydrogel properties and enzyme kinetics.
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