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Genome analysis of enterovirus 71 strains differing in mouse pathogenicity
Peng Li1,2, Yingying Yue2, Nannan Song2
1College of Life Science, Shandong Normal University, 88 East Wenhua Road, Jinan, 250014, People's Republic of China.
Virus Genes
|January 20, 2016
Summary
Enterovirus 71 (EV71) genomic analysis reveals key mutations linked to hand, foot, and mouth disease (HFMD) severity. A specific mutation in the 3C proteinase may determine EV71 virulence.
Area of Science:
- Virology
- Genomics
- Molecular Biology
Background:
- Enterovirus 71 (EV71) causes hand, foot, and mouth disease (HFMD), sometimes leading to severe neurological complications.
- Understanding EV71 virulence factors is crucial but remains limited.
- Genomic information is key to deciphering EV71 virulence.
Purpose of the Study:
- To investigate the relationship between EV71 genomic sequences and virulence.
- To identify genetic differences in EV71 strains causing mild versus severe HFMD.
Main Methods:
- Full-length genomic sequencing of six EV71 strains from HFMD patients.
- Infection characteristic analysis using a one-day-old BALB/c mouse model.
- Comparative analysis of nucleotide and amino acid sequences.
Main Results:
- All analyzed EV71 strains belonged to subgenogroup C4a.
- Forty nucleotide differences were identified between high and low virulence strains.
- A specific amino acid mutation (Asn1617 → Asp1617) in the 3C proteinase was linked to virulence.
Conclusions:
- EV71 virulence is associated with specific genomic variations.
- A mutation in the 3C proteinase active site may be a key virulence determinant.
- Further research into EV71 3C proteinase is warranted.
