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Related Concept Videos

Formation of Lipopolysaccharides01:19

Formation of Lipopolysaccharides

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Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin,...
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Sperm Structure and Semen Composition01:22

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During ejaculation, males release around 2-5 milliliters of semen, which is a complex mixture of mature sperm and various fluids produced by accessory glands. The mature sperm cells measure approximately 60 micrometers in length and consist of a head, neck, midpiece, and tail. The head is flattened and tapered, measuring about 4 to 5 micrometers in length. It contains a nucleus with condensed chromosomes and an acrosome, a cap-like structure filled with enzymes essential for penetrating the...
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Medium-throughput Screening Assays for Assessment of Effects on Ca2+-Signaling and Acrosome Reaction in Human Sperm
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Lipopolysaccharide Compromises Human Sperm Function by Reducing Intracellular cAMP.

Zhongyuan Li1, Dahu Zhang, Yuanqiao He

  • 1Department of Urology, Xiangyang Hospital, Hubei University of Medicine.

The Tohoku Journal of Experimental Medicine
|January 20, 2016
PubMed
Summary
This summary is machine-generated.

Lipopolysaccharide (LPS) from Gram-negative bacteria impairs human sperm motility in vitro by reducing intracellular cyclic adenosine monophosphate (cAMP) levels. This study clarifies LPS

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Area of Science:

  • Reproductive Biology
  • Toxicology
  • Microbiology

Background:

  • Global decline in human semen quality necessitates understanding environmental impacts.
  • Lipopolysaccharide (LPS), an endotoxin from Gram-negative bacteria, is known to affect sperm production and quality in vivo.
  • The in vitro effects of LPS on mature human sperm function remain largely uncharacterized.

Purpose of the Study:

  • To investigate the in vitro toxicity of LPS on human sperm function.
  • To elucidate the underlying molecular mechanisms of LPS-induced effects on sperm.

Main Methods:

  • Human sperm were incubated with varying concentrations of LPS (0.1-100 μg/ml) for 1-12 hours.
  • Assessed sperm viability, motility (total and progressive), capacitation, and acrosome reaction.
  • Measured intracellular concentrations of cyclic adenosine monophosphate (cAMP) and calcium ([Ca(2+)]i), and protein-tyrosine phosphorylation.

Main Results:

  • LPS significantly inhibited sperm total and progressive motility in a dose-dependent manner.
  • Sperm viability, capacitation, and acrosome reaction were not affected by LPS exposure.
  • LPS decreased intracellular cAMP levels dose-dependently, but did not alter [Ca(2+)]i or protein-tyrosine phosphorylation.

Conclusions:

  • In vitro exposure to LPS negatively impacts human sperm motility.
  • The primary mechanism involves a dose-dependent reduction in intracellular cAMP.
  • LPS does not affect key sperm functions like viability, capacitation, or acrosome reaction in vitro.