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In humans, the five forms of alpha chains are LAMA 1, LAMA 2, LAMA 3, LAMA 4, and LAMA 5. The four forms of beta chains are LAMB 1, LAMB 2, LAMB 3, and LAMB 4. The three forms of gamma...
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A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis
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Lamina Associated Polypeptide 1 (LAP1) Interactome and Its Functional Features.

Joana B Serrano1, Odete A B da Cruz E Silva2, Sandra Rebelo3

  • 1Neuroscience and Signalling Laboratory, Department of Medical Sciences, Institute of Biomedicine-iBiMED, University of Aveiro, 3810-193 Aveiro, Portugal. jmbs@ua.pt.

Membranes
|January 20, 2016
PubMed
Summary

Lamina-associated polypeptide 1 (LAP1) protein interactions reveal its role in nuclear envelope structure and DNA repair pathways. Further analysis suggests novel functions in cell morphology and organization.

Keywords:
CytoscapeGO terms enrichment analysisGeneMANIAIngenuity pathway analysisInner nuclear membraneLamina associated polypeptidedatabaseinteractorsnetworknuclear envelope

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Lamina-associated polypeptide 1 (LAP1) is an inner nuclear membrane protein crucial for nuclear envelope structure.
  • LAP1's exact cellular functions and pathway associations remain largely uncharacterized.

Purpose of the Study:

  • To identify and curate the protein interactome of LAP1.
  • To elucidate LAP1's putative functions and cellular pathway associations through interactome analysis.

Main Methods:

  • Utilized public databases to identify LAP1 interacting proteins.
  • Performed curated analysis of the LAP1 interactome.
  • Employed Ingenuity Pathway Analysis (IPA) for functional and pathway enrichment.

Main Results:

  • Identified 41 interactions involving LAP1.
  • Key interactors include TRF2, TERF2IP, RIF1, ATM, MAD2L1, and MAD2L1BP, supporting roles in DNA repair and nuclear organization.
  • IPA revealed enrichment in "Telomerase signalling" and "Telomere Extension by Telomerase" pathways.

Conclusions:

  • LAP1 interactome analysis reinforces its role in nuclear envelope structure and DNA repair.
  • Novel functions for LAP1 are suggested in cell morphology, cellular assembly, organization, and DNA replication/recombination/repair.