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Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
Published on: August 25, 2018
A novel method to assess the biodiversity of parasites using 18S rDNA Illumina sequencing; parasitome analysis method
Akina Hino1, Haruhiko Maruyama1, Taisei Kikuchi1
1Division of Parasitology, Faculty of Medicine, University of Miyazaki, Miyazaki 889-1692, Japan.
Abstract:
Understanding parasite diversity has important implications in several research fields, including ecology, evolutionary biology, and epidemiology. Here, we introduce a novel method to assess the biodiversity of parasites-especially those in the host alimentary tract-using an 18S rDNA-based metagenomic approach. The method is easy and quick compared to conventional methods, and does not require dissections of host bodies or identification skills for various parasite species. The use of a "next generation sequencer" in this method allows us to perform the assessment in a high throughput manner, which will increase our knowledge of parasite diversity.
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