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Phenotypic characterization of nanshi oral liquid alters metabolic signatures during disease prevention
Aihua Zhang1,2, Qi Liu1,2, Hongwei Zhao3
1National TCM Key Laboratory of Serum Pharmacochemistry, Laboratory of Metabolomics, Heilongjiang University of Chinese Medicine, Heping Road 24, Harbin 150040, China.
Abstract:
This paper was designed to investigate the phenotypic characterization of Nanshi Oral Liquid (NOL) alters metabolic signatures of the 'Kidney Yang Deficiency syndrome' (KYDS). Urine metabolites were profiled by UPLC-ESI-Q-TOF-HDMS. The significantly changed metabolites such as xanthurenic acid, 4,8-dihydroxyquinoline, 3-methyldioxyindole, 4,6-dihydroxyquinoline, kynurenic acid, hippuric acid, taurine, tyramine, and 3-metanephrine, had been identified, and were related to the disturbance in tyrosine metabolism, steroid hormone biosynthesis, taurine and hypotaurine metabolism, tryptophan metabolism, phenylalanine metabolism and lysine degradation, which were helpful to further understanding the KYDS and intervention mechanism of NOL. The biochemical result showed that NOL can alleviate the kidney impairment induced by KYDS. Metabolomics results indicated the significantly changed metabolites were found to be reasonable in explaining the action mechanism of NOL. Interestingly, the effectiveness of NOL against KYDS was proved using the established metabolomics method and regulated the biomarkers as well as adjusted the metabolic disorder pathways. NOL had potentially pharmacological effect through regulating multiple perturbed pathways to normal state. This work showed that the metabolomics method was a powerful approach for studying the phenotypic characterization of disease's syndrome during disease prevention and its intervention mechanism.
Insights
Nanshi Oral Liquid (NOL) effectively treats Kidney Yang Deficiency syndrome (KYDS) by restoring metabolic balance. This study utilized metabolomics to identify key biomarkers and pathways affected by KYDS, demonstrating NOL's therapeutic potential.
Area of Science:
- Metabolomics
- Traditional Chinese Medicine
- Biochemistry
Background:
- Kidney Yang Deficiency syndrome (KYDS) is characterized by specific metabolic alterations.
- Nanshi Oral Liquid (NOL) is a traditional remedy with potential therapeutic effects on KYDS.
- Understanding the molecular mechanisms of KYDS and NOL intervention is crucial.
Purpose of the Study:
- To investigate the phenotypic characterization of KYDS using metabolomics.
- To elucidate the intervention mechanism of NOL on KYDS metabolic signatures.
- To validate the efficacy of NOL in alleviating KYD-induced kidney impairment.
Main Methods:
- Urine metabolite profiling using Ultra-Performance Liquid Chromatography-Electrospray Ionization-Quadrupole-Time-of-Flight-High Definition Mass Spectrometry (UPLC-ESI-Q-TOF-HDMS).
- Identification and quantification of significantly altered metabolites.
- Analysis of metabolic pathways affected by KYDS and NOL treatment.
Main Results:
- Identified key altered metabolites including xanthurenic acid, kynurenic acid, hippuric acid, and taurine.
- Discovered metabolic disturbances in tyrosine, tryptophan, and steroid hormone biosynthesis pathways.
- Demonstrated that NOL alleviates kidney impairment and normalizes perturbed metabolic pathways in KYDS.
Conclusions:
- Metabolomics is a powerful tool for characterizing disease phenotypes and intervention mechanisms.
- NOL exerts pharmacological effects by regulating multiple metabolic pathways.
- This study provides a scientific basis for the use of NOL in treating KYDS.
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