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Updated: Mar 26, 2026
A Rapid In Vivo Bioassay for Developmentally Active Enhancers
Knockdown of Lmo7 inhibits chick myogenesis
Ana C B Possidonio1, Carolina P Soares1, Marcio Fontenele2
1Laboratório de Diferenciação Muscular e Citoesqueleto, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, RJ, Brazil.
Abstract:
The multifunctional protein Lmo7 has been implicated in some aspects of myogenesis in mammals. Here we studied the distribution and expression of Lmo7 and the effects of Lmo7 knockdown in primary cultures of chick skeletal muscle cells. Lmo7 was localized within the nuclei of myoblasts and at the perinuclear region of myotubes. Knockdown of Lmo7 using siRNA specific to chick reduces the number and width of myotubes and the number of MyoD positive-myoblasts. Both Wnt3a enriched medium and Bio, activators of the Wnt/beta-catenin pathway, could rescue the effects of the Lmo7 knockdown suggesting a crosstalk between the Wnt/beta-catenin and Lmo7-mediated signaling pathways. Our data shows a role of Lmo7 during the initial events of chick skeletal myogenesis, particularly in myoblast survival.
Insights
The LIM-only protein 7 (Lmo7) is crucial for skeletal muscle development in chicks. Lmo7 knockdown impairs myoblast survival and myotube formation, indicating its vital role in myogenesis.
Area of Science:
- Muscle Biology
- Developmental Biology
- Cell Signaling
Background:
- The multifunctional protein Lmo7 is implicated in mammalian myogenesis.
- Its specific role in avian skeletal muscle development requires further investigation.
Purpose of the Study:
- To investigate the distribution and expression of Lmo7 in chick skeletal muscle cells.
- To determine the effects of Lmo7 knockdown on myogenesis in vitro.
- To explore potential crosstalk between Lmo7 and the Wnt/beta-catenin pathway.
Main Methods:
- Primary cultures of chick skeletal muscle cells were utilized.
- Lmo7 expression and localization were examined.
- Lmo7 knockdown was performed using chick-specific siRNA.
- Wnt/beta-catenin pathway activators (Wnt3a and Bio) were used for rescue experiments.
Main Results:
- Lmo7 localizes to the nuclei of myoblasts and the perinuclear region of myotubes.
- Lmo7 knockdown reduced myotube number and width, and decreased MyoD-positive myoblasts.
- Wnt3a and Bio treatments rescued the effects of Lmo7 knockdown.
Conclusions:
- Lmo7 plays a significant role in the early stages of chick skeletal myogenesis.
- Lmo7 is particularly important for myoblast survival.
- A crosstalk exists between Lmo7-mediated signaling and the Wnt/beta-catenin pathway during myogenesis.
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