Related Experiment Video
Updated: Mar 26, 2026

Evaluation of Microbial Safety of Dairies using Bacterial Proteomic Profiling via MALDI Approach
Published on: October 7, 2025
Proteomic profiling of an undefined microbial consortium cultured in fermented dairy manure: Methods development
Andrea J Hanson1, Andrzej J Paszczynski2, Erik R Coats3
1Microbiology, Molecular Biology, and Biochemistry Graduate Program, Department of Biological Sciences, University of Idaho, Moscow, ID, USA.
Abstract:
The production of polyhydroxyalkanoates (PHA; bioplastics) from waste or surplus feedstocks using mixed microbial consortia (MMC) and aerobic dynamic feeding (ADF) is a growing field within mixed culture biotechnology. This study aimed to optimize a 2DE workflow to investigate the proteome dynamics of an MMC synthesizing PHA from fermented dairy manure. To mitigate the challenges posed to effective 2DE by this complex sample matrix, the bacterial biomass was purified using Accudenz gradient centrifugation (AGC) before protein extraction. The optimized 2DE method yielded high-quality gels suitable for quantitative comparative analysis and subsequent protein identification by LC-MS/MS. The optimized 2DE method could be adapted to other proteomic investigations involving MMC in complex organic or environmental matrices.
More Related Videos
06:58Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems
Published on: August 23, 2019
09:52A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease
Published on: January 10, 2025
Related Concept Videos
Microbes in Food Production
Methods to Assess Microbial Communities
Microbes in the Production of Fermented Foods
Microbial Fermentation