Fingerprint Analysis of Desmodium Triquetrum L. Based on Ultra Performance Liquid Chromatography with Photodiode...

Area of Science:

• Pharmacognosy
• Analytical Chemistry
• Phytochemistry

Background:

• Desmodium triquetrum L. is a widely used herbal medicine.
• Ensuring the quality of herbal medicines is crucial for clinical efficacy and safety.
• Standardized quality control methods are needed for D. triquetrum.

Purpose of the Study:

• To develop and validate an ultra-high performance liquid chromatography (UPLC) fingerprinting method for Desmodium triquetrum L. quality control.
• To assess the quality of D. triquetrum raw material samples using chemometric methods.

Main Methods:

• Ultra-high performance liquid chromatography with a photodiode array detector (UPLC-PDA) was employed.
• A Waters ACQUITY UPLC BEH shield RP18 column was used with a gradient mobile phase.
• Method validation included reproducibility and efficiency assessments.
• Chemometric methods such as similarity analysis, hierarchical clustering analysis, and principal component analysis were utilized.

Main Results:

• A robust UPLC fingerprinting method was successfully developed for D. triquetrum.
• The method demonstrated good reproducibility and efficiency, with analysis completed within 13 minutes.
• Quality assessment using chemometrics provided insights into sample variations.

Conclusions:

• The developed UPLC fingerprinting method is suitable for the quality control of Desmodium triquetrum L.
• This approach can aid in ensuring the consistency and quality of the herbal medicine.
• Further application of this method can support the standardization of D. triquetrum in clinical practice.