Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multicentre international observational study on airway management for anaesthesia: the STARGATE study protocol.

BMJ open·2026
Same author

Guidelines on strategies for the universal implementation of videolaryngoscopy.

European journal of anaesthesiology·2025
Same author

Haemophagocytic lymphohistiocytosis secondary to disseminated tuberculosis in a young adult with Crohn's disease.

The Medical journal of Australia·2023
Same author

Continuity and change in drug treatment: Regimes of control in Japan.

The International journal on drug policy·2023
Same author

Drug policy goals in a changing landscape: Perspectives of practitioners in Japan.

The International journal on drug policy·2023
Same author

Diagnostic pathways for breast cancer in 10 International Cancer Benchmarking Partnership (ICBP) jurisdictions: an international comparative cohort study based on questionnaire and registry data.

BMJ open·2022
Same journal

Proteomic Profiling of Endothelial Cells Under Laminar Shear Stress Confirms the Importance of KLF4 in the Regulation of Membrane Protein Expression Compared to Oscillatory Flow.

Journal of proteome research·2026
Same journal

Identification of Age-Associated Circulating Proteins and Lipids in 3800 Comorbidity-Enriched Older Adults from Japan-Based Cohorts Using Olink Assays and MRM Mass Spectrometry.

Journal of proteome research·2026
Same journal

Molecular Solution to the Paradox of Ancient Brain Preservation.

Journal of proteome research·2026
Same journal

From Method-Defined Signals to Reference Measurement Procedures: Two Decades of Mass Spectrometry-Based ProGRP Quantification.

Journal of proteome research·2026
Same journal

Proteomic Profiling of Extracellular Vesicle-Enriched Plasma Using Mag-Net for Biomarker Discovery in Pancreatic Ductal Adenocarcinoma.

Journal of proteome research·2026
Same journal

Computationally Efficient Bayesian Estimation of Graphical Networks for Omics Data.

Journal of proteome research·2026
See all related articles

Related Experiment Video

Updated: Mar 26, 2026

A Quantitative Glycomics and Proteomics Combined Purification Strategy
11:38

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

15.6K

Universal Solid-Phase Reversible Sample-Prep for Concurrent Proteome and N-Glycome Characterization.

Hui Zhou1, Samantha Morley1, Stephen Kostel1

  • 1Department of Urology, Boston Children's Hospital and Harvard Medical School , 300 Longwood Avenue, Boston, Massachusetts 02115, United States.

Journal of Proteome Research
|January 22, 2016
PubMed
Summary
This summary is machine-generated.

A new solid-phase reversible sample-prep (SRS) platform allows rapid, concurrent proteome and N-glycome analysis. This method efficiently isolates proteins, aiding cancer research and biomarker discovery.

Keywords:
N-glycomeglycoproteinmembrane proteinplasmaproteomereversible solid phasesample-prepurine

More Related Videos

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
10:59

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

1.9K
A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
09:16

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

Published on: May 4, 2022

2.8K

Related Experiment Videos

Last Updated: Mar 26, 2026

A Quantitative Glycomics and Proteomics Combined Purification Strategy
11:38

A Quantitative Glycomics and Proteomics Combined Purification Strategy

Published on: March 8, 2016

15.6K
Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
10:59

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

1.9K
A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues
09:16

A Spin-Tip Enrichment Strategy for Simultaneous Analysis of N-Glycopeptides and Phosphopeptides from Human Pancreatic Tissues

Published on: May 4, 2022

2.8K

Area of Science:

  • Biochemistry
  • Proteomics
  • Glycomics

Background:

  • Concurrent proteome and N-glycome analysis is crucial for understanding complex biological processes.
  • Existing sample preparation methods can be time-consuming and may not efficiently handle diverse sample types.

Purpose of the Study:

  • To introduce a novel solid-phase reversible sample-preparation (SRS) platform.
  • To enable rapid and concurrent characterization of proteomes and N-glycomes from various biological samples.

Main Methods:

  • Development of a functionalized silica-based bead with selective affinity for proteins.
  • Utilizing size-based separation for efficient removal of small molecules and peptides.
  • Application of SRS to diverse samples including cell lysates, tissues, and urine.
  • Coupling SRS with quantitative proteomics and N-glycomics.

Main Results:

  • SRS demonstrated high-capacity protein binding and efficient removal of contaminants.
  • The platform was successfully applied to glycoproteins, cell lysates, murine tissues, and human urine.
  • Distinct proteomic and N-glycomic alterations were identified between DU145 prostate cancer cells and DIAPH3-silenced counterparts.
  • Up-regulation of EPHA2 was observed in DIAPH3-silenced cells, suggesting a link to metastasis.

Conclusions:

  • The SRS platform offers a rapid and versatile solution for simultaneous proteomic and N-glycomic analysis.
  • SRS facilitates in-depth investigation of cellular changes, such as those related to cancer progression.
  • Findings suggest potential links between DIAPH3, EPHA2, and glycosyltransferase networks in prostate cancer metastasis.