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Published on: June 14, 2024
Investigating the Endobacteria Which Thrive in Arbuscular Mycorrhizal Fungi
Alessandro Desirò1,2, Alessandra Salvioli3, Paola Bonfante3
1Department of Life Sciences and Systems Biology, University of Turin, Viale Mattioli 25, Turin, 48824, Italy. desiro.alessandro@gmail.com.
Abstract:
The study of the so-called unculturable bacteria is still considered a challenging task. However, given recent improvements in the sensitivity of culture-free approaches, the identification and characterization of such microbes in complex biological samples is now possible. In this chapter we report how endobacteria thriving inside arbuscular mycorrhizal fungi (AMF), which are themselves obligate biotrophs of plants, can be studied using a combination of in vitro culture, molecular biology, and microscopy techniques.
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