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Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

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In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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FISH - Fluorescent In-situ Hybridization02:07

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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Reporter Genes

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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DNA Microarrays02:34

DNA Microarrays

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Related Experiment Video

Updated: Mar 26, 2026

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
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Targeted Gene Capture by Hybridization to Illuminate Ecosystem Functioning.

Céline Ribière1, Réjane Beugnot2, Nicolas Parisot3

  • 1EA 4678, CIDAM, Clermont Université, Université d'Auvergne, BP 10448, 63000, Clermont-Ferrand, France. celine.ribiere1@udamail.fr.

Methods in Molecular Biology (Clifton, N.J.)
|January 22, 2016
PubMed
Summary

This study introduces a solution hybrid selection (SHS) method to enrich target biomarkers from microbial samples. This approach helps reduce complexity, enabling a better understanding of microbial community functions.

Keywords:
MetagenomicsMetatranscriptomicsMicrobial diversityNext-generation sequencingRNA probesSolution hybrid selection

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Area of Science:

  • Microbiology
  • Metagenomics
  • Molecular Biology

Background:

  • Microbial communities are vital for ecosystem functioning but are highly complex.
  • Next-generation sequencing (NGS) has advanced microbial diversity studies.
  • Understanding microbial functions requires reducing biological complexity.

Purpose of the Study:

  • To present a novel method for selective enrichment of target-specific biomarkers.
  • To enable better understanding of microbial community structure and function.
  • To facilitate analysis of metagenomic and metatranscriptomic data.

Main Methods:

  • Utilizing solution hybrid selection (SHS) for targeted biomarker enrichment.
  • Developing sensitive, specific, and explorative RNA probes.
  • Capturing large DNA or RNA fragments containing biomarkers of interest.

Main Results:

  • Demonstrated a promising approach for selective biomarker enrichment.
  • Highlighted the critical role of probe design for method success.
  • Enabled potential linkage between microbial community structure and function.

Conclusions:

  • The SHS method offers a powerful tool for analyzing complex microbial communities.
  • Effective probe design is essential for capturing target gene repertoires.
  • This technique advances the study of microbial ecology and function.