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Updated: Mar 26, 2026

Isolation of Soil Microorganisms Using iChip Technology
Published on: January 10, 2025
Hybridization of Environmental Microbial Community Nucleic Acids by GeoChip
Joy D Van Nostrand1, Huaqin Yin1, Liyou Wu1
1Institute for Environmental Genomics and Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, USA.
Abstract:
Functional gene arrays, like the GeoChip, allow for the study of tens of thousands of genes in a single assay. The GeoChip array (5.0) contains probes for genes involved in geochemical cycling (N, C, S, and P), metal homeostasis, stress response, organic contaminant degradation, antibiotic resistance, secondary metabolism, and virulence factors as well as genes specific for fungi, protists, and viruses. Here, we briefly describe GeoChip design strategies (gene selection and probe design) and discuss minimum quantity and quality requirements for nucleic acids. We then provide detailed protocols for amplification, labeling, and hybridization of samples to the GeoChip.
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