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Updated: Mar 26, 2026

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

QconCAT: Internal Standard for Protein Quantification.

Kerry Bauer Scott¹, Illarion V Turko¹, Karen W Phinney²

¹Institute for Bioscience and Biotechnology Research, National Institute of Standards and Technology and the University of Maryland, Rockville, Maryland, USA.

Methods in Enzymology

|January 22, 2016

Summary

This summary is machine-generated.

Stable isotope labeling-mass spectrometry uses labeled internal standards for accurate protein quantification. This study details the QconCAT (concatenated peptides) strategy for precise protein abundance measurements in proteomics and clinical studies.

Keywords:

Mass spectrometry Protein quantification Proteomics Protocol QconCAT Stable isotope labeling

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Last Updated: Mar 26, 2026

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Published on: August 17, 2015

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Area of Science:

Proteomics
Analytical Chemistry
Biotechnology

Background:

Accurate protein quantification is crucial for proteomics, biomarker discovery, and clinical diagnostics.
Stable isotope labeling coupled with mass spectrometry is a powerful quantitative technique.
Existing methods require robust internal standards for reliable measurements.

Purpose of the Study:

To describe a protocol for the QconCAT (concatenated peptides) strategy.
To enable accurate and precise protein quantification using stable isotope labeling-mass spectrometry.
To provide a versatile internal standard platform for diverse biological applications.

Main Methods:

Design and in silico validation of QconCAT constructs.
Heterologous expression and purification of QconCAT standards.
Characterization of QconCAT integrity and labeling efficiency.
Application of QconCAT in mass spectrometry-based protein quantification.

Main Results:

Demonstrated successful design, expression, and purification of QconCAT standards.
Validated QconCAT as a reliable internal standard for absolute protein quantification.
Showcased the broad applicability of QconCAT in various research settings.

Conclusions:

The QconCAT strategy offers a robust and efficient platform for protein quantification.
This method significantly enhances the accuracy and precision of mass spectrometry-based proteomics.
QconCAT is a valuable tool for biomarker discovery, validation, and clinical applications.