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Updated: Mar 26, 2026
Confocal Time Lapse Imaging as an Efficient Method for the Cytocompatibility Evaluation of Dental Composites
Published on: November 9, 2014
Correlation of elution and sensitivity of cell lines to dental composites
Anand V Susila1, Venkatesh Balasubramanian1
1Rehabilitation Bio Engineering Group, Engineering Design Department, IITM, Chennai 600036, India.
Objectives:
The aim of the present study was to understand the role played by composition on the elution of dental composites and the subsequent cytotoxicity, the sensitivity of different cell lines to eluates on a temporal basis and the correlation of the two parameters elution and cytotoxicity.
Methods:
LC-MS was done to analyze the eluates. MTT assay was done to assess cytotoxicity on two cell lines.
Results:
Eluates were found to have matrix monomers, photoinitiators and their degradation products. The short-term viability of other mammalian cell line was inferior to human cell line. However human cell line became more sensitive to long-term incubation with composites. There was a strong inverse correlation to elution of monomers and photoinitiators and cell viability for both cell lines. Bisphenol A elution did not correlate to cell viability.
Significance:
Other mammalian cell lines may be more sensitive to acute toxin build-up than human cell line while the latter may be more sensitive to prolonged toxin exposure. Dimethacrylate based composites elute more and exert strong cytotoxicity than Ormocer and Silorane based composites. Most of the eluates correlated linearly to cytotoxicity.
