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Identification of an interaction between EI and a histidine kinase-response regulator hybrid protein in Gluconobacter
Shan Li1, Yushu Ma1, Dongzhi Wei1
1State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China.
Abstract:
Gluconobacter oxydans may contain an incomplete phosphoenolpyruvate: carbohydrate phosphotransferase system consisting of three components--EI, HPr and EIIA, while the function of individual members of the system remains unknown. In this research, a specific interaction between EI and a histidine kinase-response regulator hybrid protein was screened by yeast two-hybrid assay, and the interaction was further identified with GST pull-down assay and bimolecular fluorescence complementation assay in vitro and in vivo, respectively. As the histidine kinase-response regulator hybrid protein serves as a member of two-component system in G. oxydans, its interaction with EI implied that PTS may play certain roles in bacteria under stress.
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