Related Experiment Video
Updated: Mar 26, 2026

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
Published on: May 18, 2017
Multidimensional Monitoring of Keratin Intermediate Filaments in Cultured Cells and Tissues
Nicole Schwarz1, Marcin Moch1, Reinhard Windoffer1
1Institute of Molecular and Cellular Anatomy, RWTH Aachen University, Aachen, Germany.
Abstract:
Keratin filaments are a hallmark of epithelial differentiation. Their cell type-specific spatial organization and dynamic properties reflect and support epithelial function. To study this interdependency, imaging of fluorescently tagged keratins is a widely used method by which the temporospatial organization and behavior of the keratin intermediate filament network can be analyzed in living cells. Here, we describe methods that have been adapted and optimized to dissect and quantify keratin intermediate filament network dynamics in vital cultured cells and functional tissues.
Related Concept Videos
Studying the Cytoskeleton
Types of Intermediate Filaments
Disassembly of Intermediate Filaments
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...

