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Mitochondrial Remodeling in Chicken Induced Pluripotent Stem-Like Cells
Hyun Woo Choi1, Jong Soo Kim1, Sol Choi1
11 Department of Animal Biotechnology, College of Animal Bioscience and Technology, Konkuk University , Seoul, Republic of Korea.
Stem Cells and Development
|January 23, 2016
Summary
Researchers successfully reprogrammed chicken somatic cells into induced pluripotent stem-like cells (ciPSLCs). These cells exhibit key pluripotency markers and a distinct cellular morphology, suggesting successful intracytoplasmic remodeling during reprogramming.
Area of Science:
- Stem cell biology
- Reproductive biology
- Cellular reprogramming
Background:
- Chicken pluripotent stem cells (PSCs) are valuable for developmental studies but difficult to culture long-term.
- Induced pluripotent stem cells (iPSCs) offer a potential solution, but the intracytoplasmic changes during chicken cell reprogramming are poorly understood.
Purpose of the Study:
- To generate and characterize chicken iPS-like cells (ciPSLCs) from somatic cells.
- To investigate the intracytoplasmic remodeling process during chicken cell reprogramming.
Main Methods:
- Generated ciPSLCs from chicken embryonic fibroblasts using retroviral delivery of human reprogramming factors.
- Maintained ciPSLCs for over 10 passages.
- Analyzed pluripotency marker expression (cNong, cSox2) and cellular morphology (nucleus-to-cytoplasm ratio, mitochondrial structure).
Main Results:
- Successfully generated ciPSLCs that expressed endogenous chicken pluripotency markers.
- ciPSLCs exhibited a higher nucleus-to-cytoplasm ratio and globular mitochondria with immature cristae.
- This morphology resembles mammalian PSCs, differing from mature avian somatic cells.
Conclusions:
- Chicken somatic cells can be reprogrammed into iPS-like cells with characteristics of pluripotency.
- Intracytoplasmic organelles undergo successful remodeling during the reprogramming of chicken cells.
- These findings advance the potential for using chicken iPSCs in research and biotechnology.
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