Transfer of foreign genes by electroporation and their expression in mammalian cells

Expression of foreign genes transferred into mammalian cells by electroporation has been studied. The pX1TK gene, pSV2Neo gene and pUCEJ oncogene have been introduced into MLTK- cells and NIH/3T3 cells, respectively. Stable transformation transient expression of TK gene by MLTK- cells as well as stable and malignant transformation of NIH/3T3 cells have been obtained. Transient expression frequency is about 80% and stable transformation frequency is about 10(-4). Integration of foreign genes into the cellular genome was verified with molecular hybridization. Tumor development was observed after inoculation of transformed cells into nude mice.