Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

17.2K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
17.2K
Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

1.8K
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...
1.8K
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

8.0K
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
8.0K
Antigen Processing Pathways01:31

Antigen Processing Pathways

2.9K
MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...
2.9K
Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

86.1K
Overview
86.1K
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

10.0K
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
10.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhanced B cell priming induces broadly neutralizing HIV-1 apex antibodies.

Nature·2026
Same author

HLA-E-restricted T cells primed by a modified HLA-B*57:01 restricted HIV-1 peptide suppress HIV-1 replication.

JCI insight·2026
Same author

Generation of a rhesus macaque harboring a multivalent reporter for assessing gene editing outcomes.

Scientific reports·2026
Same author

Distinct phases of immune system programming during ART-suppressed immunodeficiency virus infection.

bioRxiv : the preprint server for biology·2026
Same author

Nonclassical MHC-I Molecules: Emerging Therapeutic Targets in Next-Generation Immunotherapy.

MedComm·2026
Same author

Shared HLA-E and Mamu-E Peptide Repertoires With Subtle Peptide Binding Differences Revealed by Combined nDSF- and Fluorescence Polarisation-Based Methods.

European journal of immunology·2026

Related Experiment Video

Updated: Mar 26, 2026

Author Spotlight: Optimized Protocol for Detecting Antigen-Specific T Cells in Mouse Lungs Using Tetramers
09:15

Author Spotlight: Optimized Protocol for Detecting Antigen-Specific T Cells in Mouse Lungs Using Tetramers

Published on: July 19, 2024

1.9K

Broadly targeted CD8⁺ T cell responses restricted by major histocompatibility complex E.

Scott G Hansen1, Helen L Wu1, Benjamin J Burwitz1

  • 1Vaccine and Gene Therapy Institute and Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR 97006, USA.

Science (New York, N.Y.)
|January 23, 2016
PubMed
Summary

Vaccination with modified cytomegalovirus vectors elicits diverse T cell responses restricted by Major Histocompatibility Complex E (MHC-E). These MHC-E-restricted CD8(+) T cell responses may offer superior efficacy against persistent viral infections.

More Related Videos

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
10:13

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

9.6K
Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
12:09

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Published on: February 28, 2019

10.4K

Related Experiment Videos

Last Updated: Mar 26, 2026

Author Spotlight: Optimized Protocol for Detecting Antigen-Specific T Cells in Mouse Lungs Using Tetramers
09:15

Author Spotlight: Optimized Protocol for Detecting Antigen-Specific T Cells in Mouse Lungs Using Tetramers

Published on: July 19, 2024

1.9K
Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
10:13

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

9.6K
Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
12:09

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Published on: February 28, 2019

10.4K

Area of Science:

  • Immunology
  • Virology
  • Structural Biology

Background:

  • Major Histocompatibility Complex E (MHC-E) is a conserved, nonclassical MHC class Ib molecule regulating natural killer (NK) cells.
  • MHC-E is upregulated by persistent viruses like HIV and simian immunodeficiency virus to evade NK cell detection.

Purpose of the Study:

  • To investigate the peptide-binding and T cell presentation capabilities of MHC-E.
  • To explore the potential of MHC-E-restricted T cell responses against persistent viral infections.

Main Methods:

  • Rhesus macaques were vaccinated with rhesus cytomegalovirus vectors lacking specific genes (Rh157.5 and Rh157.4).
  • MHC-E-restricted presentation of peptide epitopes to CD8αβ(+) T cells was analyzed.
  • Computational structural analysis was performed on MHC-E.

Main Results:

  • Vaccination induced MHC-E-restricted presentation of diverse peptide epitopes to CD8αβ(+) T cells.
  • Approximately 4 distinct epitopes per 100 amino acids were presented across tested antigens.
  • Structural analysis revealed a stable, open MHC-E binding groove accommodating diverse side-chain interactions.

Conclusions:

  • MHC-E presents a broad repertoire of epitopes, suggesting its potential as a target for immune interventions.
  • Exploiting MHC-E-restricted CD8(+) T cell responses could leverage viral immune-evasion strategies for enhanced therapeutic efficacy against persistent viruses.