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Clonal Relatedness among Imipenem-Resistant Pseudomonas aeruginosa Isolated from ICU-Hospitalized Patients

Hamid Vaez1, Sharareh Moghim1, Bahram Nasr Esfahani1

  • 1Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Critical Care Research and Practice
|January 23, 2016
PubMed

Insights

Imipenem-resistant Pseudomonas aeruginosa is spreading in hospitals, showing high genetic diversity and resistance to common antibiotics. This suggests infection control failures and poses a significant threat to patients.

Area of Science:

  • Medical Microbiology
  • Infectious Diseases
  • Healthcare Epidemiology

Background:

  • Imipenem-resistant Pseudomonas aeruginosa (P. aeruginosa) is a growing global healthcare concern.
  • Effective infection control is crucial in intensive care units (ICUs) to prevent the spread of resistant bacteria.

Purpose of the Study:

  • To investigate the clonal spread of imipenem-resistant P. aeruginosa in ICU-hospitalized patients.
  • To determine the antibiotic resistance patterns and genetic diversity of these isolates.

Main Methods:

  • Analysis of 150 wound specimens from ICU patients.
  • Antibiotic resistance profiling using Kirby-Bauer's disk diffusion.
  • Clonal diversity assessment via Random Amplified Polymorphic DNA- (RAPD-) PCR.
  • Detection of Metallo-beta-lactamase (MBL) production using the ethylenediaminetetraacetic acid (EDTA) combined disk test.

Main Results:

  • 100% resistance to imipenem and meropenem; 90% resistance to ciprofloxacin and ceftazidime.
  • No resistance observed against polymyxin B.
  • 40% of isolates were MBL-positive.
  • High genetic diversity detected with 37 distinct RAPD types, indicating cross-acquisition.

Conclusions:

  • Significant clonal spread of imipenem-resistant P. aeruginosa in the hospital setting.
  • High prevalence of MBL-producing isolates represents a substantial threat to hospitalized patients.
  • Findings suggest potential failures in current infection control practices.

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