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Updated: Mar 26, 2026

Imaging and Analysis for Quantifying Maize (Zea mays) Abiotic Stress Phenotypes
Published on: March 28, 2025
Multivariate Analysis of Grain Yield and Its Attributing Traits in Different Maize Hybrids Grown under Heat and
Fawad Ali1, Naila Kanwal1, Muhammmad Ahsan1
1Department of Plant Breeding and Genetics, University of Agriculture Faisalabad, Faisalabad 38040, Pakistan.
Abstract:
This study was carried out to evaluate F1 single cross-maize hybrids in four crop growing seasons (2010-2012). Morphological traits and physiological parameters of twelve maize hybrids were evaluated (i) to construct seed yield equation and (ii) to determine grain yield attributing traits of well-performing maize genotype using a previously unexplored method of two-way hierarchical clustering. In seed yield predicting equation photosynthetic rate contributed the highest variation (46%). Principal component analysis data showed that investigated traits contributed up to 90.55% variation in dependent structure. From factor analysis, we found that factor 1 contributed 49.6% variation (P < 0.05) with primary important traits (i.e., number of leaves per plant, plant height, stem diameter, fresh leaves weight, leaf area, stomata conductance, substomata CO2 absorption rate, and photosynthetic rate). The results of two-way hierarchical clustering demonstrated that Cluster III had outperforming genotype H12 (Sultan × Soneri) along with its most closely related traits (photosynthetic rate, stomata conductance, substomata CO2 absorption rate, chlorophyll contents, leaf area, and fresh stem weight). Our data shows that H12 (Sultan × Soneri) possessed the highest grain yield per plant under environmentally stress conditions, which are most likely to exist in arid and semiarid climatic conditions, such as in Pakistan.
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