Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dopaminergic manipulation modulates frequency-specific EEG connectivity patterns: evidence from a single dose drug challenge study.

Frontiers in neuroscience·2026
Same author

Hydrophobic Refinement of Polarity-Switchable Lipo-Xenopeptides Modulates Endosomal Escape and Enhances mRNA Delivery In Vitro and In Vivo.

Journal of the American Chemical Society·2026
Same author

Beyond sequence similarity: ML-powered identification of pHLA off-targets for TCR-mimic antibodies using high throughput binding kinetics.

mAbs·2025
Same author

Less Than Zero?

Experimental psychology·2025
Same author

Endothelial Sprout Formation Is Regulated by Substrate Stiffness and Notch Signaling.

International journal of molecular sciences·2025
Same author

Homoharringtonine (omacetaxine mepesuccinate) limits the angiogenic capacity of endothelial cells and reorganises filamentous actin.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2025
Same journal

Fiber-reinforced hydrogels: From multiscale structural design to advanced engineering applications.

Biointerphases·2026
Same journal

Development and validation of a low-cost, direct-current-based biosensor for real-time monitoring of transendothelial electrical resistance in cell barriers.

Biointerphases·2026
Same journal

Biointerfaces in India.

Biointerphases·2026
Same journal

Biomimetic illumination enhancement inspired by guanine platelets in the photophore surface of the deep-sea bristlemouth Sigmops gracilis.

Biointerphases·2026
Same journal

Binding and orientation of ice nucleating proteins on hydrophilic and hydrophobic surfaces probed by photoelectron spectroscopies.

Biointerphases·2026
Same journal

Shell damage and mandible mechanics in the ant Messor wasmanni.

Biointerphases·2026
See all related articles

Related Experiment Video

Updated: Mar 26, 2026

Pattern Generation for Micropattern Traction Microscopy
09:26

Pattern Generation for Micropattern Traction Microscopy

Published on: February 17, 2022

2.8K

Versatile method to generate multiple types of micropatterns.

Felix Jakob Segerer1, Peter Johan Friedrich Röttgermann1, Simon Schuster2

  • 1Faculty of Physics and Center for NanoScience, Ludwig-Maximilians-Universität München, Geschwister-Scholl-Platz 1, D-80539 Munich, Germany.

Biointerphases
|January 24, 2016
PubMed
Summary
This summary is machine-generated.

Researchers developed a versatile micropatterning technique for cell behavior studies. This flexible method allows controlled protein patterns on diverse substrates, enabling complex microenvironment creation for cell science experiments.

More Related Videos

Micropunching Lithography for Generating Micro- and Submicron-patterns on Polymer Substrates
09:24

Micropunching Lithography for Generating Micro- and Submicron-patterns on Polymer Substrates

Published on: July 2, 2012

15.7K
Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates
09:30

Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates

Published on: June 2, 2022

3.0K

Related Experiment Videos

Last Updated: Mar 26, 2026

Pattern Generation for Micropattern Traction Microscopy
09:26

Pattern Generation for Micropattern Traction Microscopy

Published on: February 17, 2022

2.8K
Micropunching Lithography for Generating Micro- and Submicron-patterns on Polymer Substrates
09:24

Micropunching Lithography for Generating Micro- and Submicron-patterns on Polymer Substrates

Published on: July 2, 2012

15.7K
Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates
09:30

Generation of Multicue Cellular Microenvironments by UV-Photopatterning of Three-Dimensional Cell Culture Substrates

Published on: June 2, 2022

3.0K

Area of Science:

  • Cell Biology
  • Biomaterials Science
  • Microfluidics and Lab-on-a-Chip Technologies

Background:

  • Micropatterning is crucial for studying cell behavior in controlled microenvironments.
  • Existing micropatterning techniques face limitations due to diverse experimental needs in substrates, coatings, and pattern complexity.
  • A need exists for flexible patterning protocols compatible with various materials and strategies for complex experimental setups.

Purpose of the Study:

  • To present a versatile and adaptable micropatterning approach for cell science.
  • To enable the creation of homogeneous and complex micropatterns on various substrates.
  • To offer control over protein density and facilitate gradient formation for specialized microenvironments.

Main Methods:

  • The protocol utilizes plasma treatment, protein coating, and a poly(L-lysine)-grafted-poly(ethylene glycol) backfill step.
  • Protein density within patterns is controllable, allowing for the formation of density gradients.
  • The method is compatible with microcontact printing for creating multi-component patterns in a single step.

Main Results:

  • The developed technique produces homogeneous micropatterns across a variety of substrates.
  • It allows for precise control over protein density and the generation of surface-bound protein gradients.
  • Combining this method with microcontact printing enables the creation of intricate, three-component patterns.

Conclusions:

  • This versatile micropatterning protocol is simple to implement in cell science laboratories.
  • It provides a flexible platform for generating diverse and complex microenvironments for cell studies.
  • The technique supports the creation of highly specialized experimental setups, advancing cell behavior research.