Hymenobacter paludis sp. nov., isolated from a marsh

A bacterial strain, designated KBP-30^T, was isolated from a water sample taken from the Banping Lake Wetland Park in Taiwan and characterized taxonomically using a polyphasic approach. Cells of strain KBP-30^T were Gram-stain-negative, aerobic, motile by gliding rods that were covered by large capsules and formed red colonies. Growth occurred at 10-37 °C (optimum 20 °C), at pH 6-8 (optimum pH 6) and with 0-1 % NaCl (optimum 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain KBP-30^T belonged to the genus Hymenobacter and was most closely related to Hymenobacter ocellatus Myx 2105^T with a sequence similarity of 97.7 %; 16S rRNA gene sequence similarities were less than 95.1 % with other members of the genus. Strain KBP-30^T contained iso-C_15 : 0, summed feature 4 (iso-C_17 : 1 I and/or anteiso-C_17 : 1 B), anteiso-C_15 : 0, iso-C_17 : 0 3-OH and summed feature 3 (C_16 : 1ω7c and/or C_16 : 1ω6c) as the predominant fatty acids. The major isoprenoid quinone was MK-7. The polar lipid profile consisted of phosphatidylethanolamine, three unidentified aminophospholipids, an unidentified aminolipid, an unidentified glycolipid and eight unidentified lipids. The major polyamine was homospermidine. The DNA G+C content of the genomic DNA was 60.3 mol%. The DNA-DNA relatedness of strain KBP-30^T with respect to Hymenobacter ocellatus Myx 2105^T was less than 42 %. On the basis of the phylogenetic inference and phenotypic data, strain KBP-30^T represents a novel species of the genus Hymenobacter, for which the name Hymenobacter paludis sp. nov. is proposed. The type strain is KBP-30^T ( = LMG 27293^T = KCTC 32237^T).