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Comparative evaluation of the WHO and DAKOPATTS enzyme-linked immunoassay kits for rotavirus detection

T H Flewett, C F Arias, L F Avendano

    Bulletin of the World Health Organization
    |January 1, 1989
    PubMed

    Insights

    Two enzyme-linked immunosorbent assay kits were compared for rotavirus detection. The DAKO-ELISA kit demonstrated comparable sensitivity and specificity to the WHO-ELISA kit in diverse laboratory settings.

    Area of Science:

    • Virology
    • Immunology
    • Clinical Diagnostics

    Background:

    • Rotavirus is a leading cause of severe diarrheal disease in infants and young children globally.
    • Accurate and accessible diagnostic tools are crucial for rotavirus surveillance and management, especially in resource-limited settings.

    Purpose of the Study:

    • To evaluate and compare the diagnostic performance of two enzyme-linked immunosorbent assay (ELISA) kits for rotavirus antigen detection in faecal samples.
    • To assess the suitability of the DAKO-ELISA kit for use in developing countries.

    Main Methods:

    • Faecal samples from 1,163 children, including 66 newborns, were analyzed using both the WHO-ELISA kit and the DAKO-ELISA kit.
    • The study involved parallel testing in laboratories across seven countries: Burma, Chile, India, Mexico, Pakistan, Sri Lanka, and the United Kingdom.

    Main Results:

    • The DAKO-ELISA kit showed an overall sensitivity of 97% and a specificity of 97% when compared to the WHO-ELISA kit.
    • In individual laboratories, the DAKO-ELISA kit exhibited a sensitivity range of 90-100% and a specificity range of 85-100%.
    • Specifically for newborn faecal samples, the DAKO-ELISA kit achieved 100% sensitivity and 98% specificity.

    Conclusions:

    • The DAKO-ELISA kit is a highly sensitive and specific diagnostic tool for detecting rotavirus antigen in faeces.
    • The DAKO-ELISA kit performs comparably to the WHO-ELISA kit, making it a viable option for rotavirus diagnostics in developing countries.

    Keywords:
    DiarrheaDiarrhea, InfantileDiseasesEvaluationExaminations And DiagnosesInternational AgenciesLaboratory Examinations And DiagnosesLaboratory ProceduresOrganizationsUnViral DiseasesWho

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