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Updated: Mar 26, 2026
Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa
Published on: March 11, 2018
Oxidative stress level in fresh ejaculate is not related to semen parameters or to pregnancy rates in cycles with
Aïda Pujol1, Albert Obradors1, Erica Esteo1
1Clínica EUGIN, Travessera de les Corts 322, 08029, Barcelona, Spain.
Purpose:
The purpose of the present study is to study the relationship between oxidative stress (OS) in semen, semen characteristics, and reproductive outcomes in oocyte donation intracytoplasmic sperm injection (ICSI) cycles.
Methods:
OS was measured in 132 semen samples.
Results:
OS levels were as follows: very high (1.5 %), high (43.2 %), low (30.3 %), and very low (25.0 %). Overall seminal parameters were as follows: volume (ml) = 4.2 (SD 2.1), concentration (millions/ml) = 61.6 (SD 59.8), motility (a+b%) = 47.4 (SD 18.0), and normal spermatozoa (%) = 8.2 (SD 5.1). Of the 101 cycles that reached embryo transfer, 55.4 % evolved in biochemical, 46.5 % in clinical, and 43.6 % in ongoing pregnancy. OS level does not relate to seminal parameters, fertilization rate, or pregnancy outcomes.
Conclusions:
OS testing by nitro blue tetrazolium (NBT) in fresh ejaculate might not be useful for all patients. Reproductive results with young oocytes and ICSI do not seem to be affected by OS-level semen.
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