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Updated: Mar 26, 2026

Investigating Intestinal Inflammation in DSS-induced Model of IBD
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Meta-omics in Inflammatory Bowel Disease Research: Applications, Challenges, and Guidelines
Mireia Valles-Colomer1, Youssef Darzi2, Sara Vieira-Silva1
1KU Leuven, Department of Microbiology and Immunology, Rega Institute, Leuven, Belgium VIB, Center for the Biology of Disease, Leuven, Belgium.
Abstract:
Meta-omics [metagenomics, metatranscriptomics, and metaproteomics] are rapidly expanding our knowledge of the gut microbiota in health and disease. These technologies are increasingly used in inflammatory bowel disease [IBD] research. Yet, meta-omics data analysis, interpretation, and among-study comparison remain challenging. In this review we discuss the role these techniques are playing in IBD research, highlighting their strengths and limitations. We give guidelines on proper sample collection and preparation methods, and on performing the analyses and interpreting the results, reporting available user-friendly tools and pipelines.
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