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KIAA0368-deficiency affects disassembly of 26S proteasome under oxidative stress condition

Kousuke Haratake1, Akitsugu Sato1, Fuminori Tsuruta1

  • 1Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan.

Journal of Biochemistry
|January 24, 2016
PubMed

Insights

Extracellular mutants 29 (Ecm29) protein deficiency prevents 26S proteasome dissociation during cellular stress, enhancing protein degradation and oxidative stress resistance in mice.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cellular stress leads to intracellular protein damage, necessitating degradation via the ubiquitin-proteasome system.
  • The proteasome, a protease complex, comprises a 20S core and regulatory activators.
  • The function of Extracellular mutants 29 (Ecm29), a protein encoded by KIAA0368, in proteasome regulation remains largely unelucidated.

Purpose of the Study:

  • To investigate the role of Ecm29 (KIAA0368) in cellular stress response.
  • To determine the impact of Ecm29 deficiency on proteasome function under stress conditions.

Main Methods:

  • Generation of KIAA0368-deficient (KIAA0368(-/-)) mice.
  • Assessment of proteasome activity and integrity under normal and stressed conditions.
  • Evaluation of protein degradation efficiency and oxidative stress resistance in KIAA0368(-/-) cells.

Main Results:

  • KIAA0368(-/-) mice exhibited normal proteasome activity and formation under non-stressed conditions.
  • Unlike wild-type cells, 26S proteasome dissociation was inhibited in KIAA0368(-/-) cells under stress.
  • Ecm29 deficiency correlated with enhanced damaged protein degradation and increased resistance to oxidative stress.

Conclusions:

  • Ecm29 plays a crucial role in the dissociation of the 26S proteasome during cellular stress.
  • Ecm29 deficiency confers a protective advantage against oxidative stress by maintaining proteasome integrity.
  • This study provides insights into the mechanism of proteasomal degradation under diverse stress conditions.
Keywords:
oxidative stressproteasomeproteasome activatorsprotein degradationubiquitin

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