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Disposable screen printed sensor for the electrochemical detection of delta-9-tetrahydrocannabinol in undiluted
Ceri Wanklyn1, Dan Burton1, Emma Enston1
1Oxtox Limited, Warren House, Mowbray Street, Stockport, SK1 3EJ UK.
Background:
Cannabis has an adverse effect on the ability to drive safely, therefore a rapid disposable test for Δ(9)-tetrahydrocannabinol (Δ(9)-THC), the psychoactive component of cannabis, is highly desirable for roadside testing.
Results:
A screen printed carbon electrode is used for the N-(4-amino-3-methoxyphenyl)-methanesulfonamide mediated detection of Δ(9)-THC in saliva. Mediator placed in an overlayer was galvanostatically oxidized and reacted with Δ(9)-THC to give an electrochemically active adduct which could be detected by chronoamperometric reduction. Detection of 25-50 ng/mL Δ(9)-THC spiked into undiluted saliva was achieved with a response time of 30 s. A trial of the sensors with four cannabis smokers showed sensitivity of 28 %, specificity of 99 % and accuracy of 52 %.
Conclusions:
Rapid electrochemical detection of Δ(9)-THC in undiluted saliva has been demonstrated using a disposable sensor, however the sensitivity is lower than acceptable. Further optimization of the assay and sensor format is required to improve the sensitivity of response to Δ(9)-THC.