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Updated: Mar 26, 2026

A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro
Published on: May 17, 2017
Epigenetic regulation in chondrocyte phenotype maintenance for cell-based cartilage repair
Li Duan1, Yujie Liang2, Bin Ma3
1Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Second People's HospitalShenzhen 518035, Guangdong Province, China; Department of Orthopedics, Shenzhen Second People's HospitalShenzhen 518035, Guangdong Province, China.
Abstract:
Loss of hyaline chondrocyte phenotype during the monolayer culture in vitro is a major obstacle for cell-based articular cartilage repair. Increasing evidence implicates an important role of the epigenetic regulation in maintaining the chondrocyte phenotype. DNA methylation, histone modifications and microRNAs have all been shown to contribute to chondrocyte dedifferentiation and hypertrophy. Moreover, the interplay among epigenetic regulators forms a complicated epigenetic network in regulating chondrocyte dedifferentiation. This review provides a detailed overview of the epigenetic regulation in maintaining the chondrocyte phenotype for chondrocyte-based cartilage repair.
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