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Updated: Mar 26, 2026

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
Published on: March 29, 2018
Relationship between enamel fluorosis severity and fluoride content.
Esperanza A Martinez-Mier1, Devin B Shone2, Christine M Buckley3
1Department of Cariology, Operative Dentistry and Dental Public Health, Indiana University School of Dentistry, 415 Lansing Street, Indianapolis IN 46236, USA.
Severely fluorosed teeth, both erupted and unerupted, show significantly higher enamel fluoride levels compared to sound teeth. This finding clarifies the link between fluoride exposure and enamel fluorosis severity.
Area of Science:
- Dental Science
- Biomineralization
- Toxicology
Background:
- Enamel fluorosis results from excessive fluoride during tooth development.
- Previous studies on enamel fluoride content and fluorosis severity have yielded inconsistent results.
Purpose of the Study:
- To compare enamel fluoride content with visually and histologically determined fluorosis severity.
Main Methods:
- 112 extracted teeth were assessed for fluorosis using the Thylstrup and Fejerskov Index and polarized light microscopy.
- Enamel fluoride content was measured using microbiopsy at depths of 30, 60, and 90 μm.
Main Results:
- Severely fluorosed erupted teeth had higher mean fluoride content at all measured depths compared to sound teeth.
- Unerupted teeth with varying degrees of fluorosis showed increased fluoride content at 30 μm, with differences at 60 and 90 μm depending on severity.
Conclusions:
- Severely fluorosed teeth, both erupted and unerupted, exhibit elevated enamel fluoride concentrations.
- Understanding enamel fluoride content aids in managing hard tissue diseases.
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