Related Experiment Video
Updated: Mar 26, 2026
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Published on: March 30, 2019
miR-206 functions as a novel cell cycle regulator and tumor suppressor in clear-cell renal cell carcinoma
Haibing Xiao1, Wei Xiao2, Jing Cao3
1Department of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; Institute of Urology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Purpose:
In this study we tried to systematically investigate the tumor suppressing microRNAs in ccRCC.
Materials And Methods:
The MTS cell viability and colony formation assay were used to systematically detect the tumor suppressing ability of down-regulated miRNAs in ccRCC. Then miR-206 expression was detected by RT-qPCR and in situ hybridization in ccRCC cell lines and clinical samples. Oligonucleotides were used to overexpress or down-regulate miR-206. MTS cell viability, EdU cell proliferation, colony formation assay, flow cytometry, Xenograft subcutaneously and orthotopic implantations were done to examine tumor suppressing effects of miR-206 in vitro and in vivo. Luciferase assay was performed to verify the precise target of miR-206.
Results:
We reviewed and experimentally analyzed the currently available miRNA expression profiles data of ccRCC and identified miR-206 as one of the most critical tumor-suppressing microRNAs in ccRCC. In addition, miR-206 inhibited ccRCC cell proliferation through inducing cell cycle arrest by directly targeting cell cycle related gene CDK4, CDK9 and CCND1.
Conclusions:
All these results suggested that miR-206 functioned as a novel cell cycle regulator and tumor suppressor in ccRCC and could be considered as a potential target for ccRCC therapy.
Insights
MicroRNA-206 (miR-206) acts as a tumor suppressor in clear cell renal cell carcinoma (ccRCC). It inhibits cancer cell proliferation by regulating cell cycle genes, offering a potential therapeutic target for ccRCC.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Clear cell renal cell carcinoma (ccRCC) is a significant health concern.
- Understanding the role of microRNAs in ccRCC pathogenesis is crucial for developing new therapies.
Purpose of the Study:
- To systematically investigate microRNAs with tumor-suppressing capabilities in ccRCC.
- To identify and characterize the function of specific microRNAs involved in ccRCC progression.
Main Methods:
- Expression profiling analysis to identify candidate microRNAs.
- In vitro assays (MTS, colony formation, EdU, flow cytometry) to assess cell proliferation and cell cycle.
- In vivo studies (xenografts) to evaluate tumor suppression.
- RT-qPCR, in situ hybridization, and luciferase assays for mechanism elucidation.
Main Results:
- miR-206 was identified as a critical tumor-suppressing microRNA in ccRCC.
- miR-206 significantly inhibited ccRCC cell proliferation and induced cell cycle arrest.
- miR-206 directly targets cell cycle-related genes CDK4, CDK9, and CCND1.
Conclusions:
- miR-206 functions as a novel cell cycle regulator and tumor suppressor in ccRCC.
- miR-206 represents a potential therapeutic target for ccRCC treatment.
Related Concept Videos
01:20The Retinoblastoma Gene
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
01:20The Retinoblastoma Gene
02:34Inhibition of Cdk Activity
01:23Negative Regulator Molecules
03:03mTOR Signaling and Cancer Progression
The mTOR pathway or the...
03:03mTOR Signaling and Cancer Progression