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Updated: Mar 26, 2026

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Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging
Published on: July 19, 2016
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Ultrasensitive In Situ Fluorescence Analysis using Modulated Fluorescence Interference Contrast at Nanostructured
Xiaopei Deng1, Siheng He2, Fan Xie1
1Biointerfaces Institute & Chemical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.
Advanced Materials (Deerfield Beach, Fla.)
|January 26, 2016
Abstract:
The precise modulation of fluorescence interference contrast is achieved by introducing a nanoscopically engineered spacer layer prepared by chemical vapor deposition (CVD) of functional polymers. These novel imaging substrates are chemically identical throughout their entire detection area, yet present patterns of nanoscale thickness. A protein binding cascade is studied in real time and in the presence of high background noise.

