Related Experiment Video
Updated: Mar 26, 2026

Engineered Vascularized Muscle Flap
Published on: January 11, 2016
Rectovaginal fistula repair using a gracilis muscle flap
Erik D Hokenstad1, Ziyad S Hammoudeh2, Nho V Tran2
1Division of Gynecologic Surgery, Mayo Clinic, 200 First St. SW, Rochester, MN, 55905, USA.
Introduction And Hypothesis:
This video demonstrates a technique for using a pedicled gracilis muscle flap to repair rectovaginal fistula.
Methods:
We present the case of a 48-year-old woman diagnosed with rectal cancer 2 years earlier. She underwent neoadjuvant chemoradiation followed by ultralow anterior resection. Six weeks after surgery, a fistula was identified at the anastomotic site. Preoperative planning with urogynecology, plastic surgery, and colon and rectal surgery teams deemed a pedicled gracilis muscle flap to be the best approach for this patient due to the rich blood supply and the patient's prior history of pelvic irradiation. The gracilis muscle is suitable due to the proximity of its vascular pedicle to the perineum, length, and minimal functional donor-site morbidity. We discuss techniques used to interpose a gracilis muscle flap between the rectum and vagina to repair a rectovaginal fistula.
Conclusion:
Using the gracilis muscle is a viable option for repairing rectovaginal fistulas, especially in the setting of prior pelvic radiation. A multispecialty approach may be beneficial in complex cases to determine the optimal approach for repair.

