Related Experiment Videos
A novel flagellar Ca2+-binding protein in trypanosomes
D M Engman1, K H Krause, J H Blumin
1Department of Biochemistry, University of Iowa, Iowa City.
The Journal of Biological Chemistry
|November 5, 1989
Summary
A 24-kDa protein from Trypanosoma cruzi, which causes Chagas' disease, binds calcium ions. This calcium-binding protein is located in the parasite's flagellum, suggesting a role in motility.
Area of Science:
- Parasitology
- Molecular Biology
- Biochemistry
Background:
- Trypanosoma cruzi causes Chagas' disease.
- A 24-kDa protein is recognized by infected hosts.
- This protein contains potential calcium-binding motifs.
Purpose of the Study:
- To investigate the calcium-binding properties of the 24-kDa Trypanosoma cruzi protein.
- To determine the protein's localization within the parasite.
Main Methods:
- Expressed the protein's cDNA in E. coli.
- Assessed calcium-binding capacity and affinity.
- Used immunofluorescence to localize the native protein.
Main Results:
- Recombinant and native proteins bind calcium (Ca2+).
- Binding affinity is high (apparent Kd < 50 microM Ca2+).
- Protein is localized to the trypanosome flagellum.
Conclusions:
- The 24-kDa protein binds calcium via E-F hand structures.
- Flagellar localization suggests a role in parasite motility.
- This protein is a potential target for Chagas' disease research.