Marinobacterium profundum sp. nov., a marine bacterium from deep-sea sediment

A Gram-stain-negative, rod-shaped and motile strain, designated PAMC 27536^T, was isolated from deep-sea sediment in the East Sea, Korea. Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Marinobacterium. Phylogenetic analyses revealed that strain PAMC 27536^T was related most closely to Marinobacterium rhizophilum CL-YJ9^T with a 16S rRNA gene sequence similarity of 98.5 % and to other members of the genus Marinobacterium (94.0-91.7 %). Genomic relatedness analyses between strain PAMC 27536^T and M. rhizophilum KCCM 42386^T gave an average nucleotide identity of 85.6 % and an estimated DNA-DNA hybridization of 24.6 % using the genome-to-genome distance calculator, indicating that they represent genomically distinct species. Cells of strain PAMC 27536^T grew optimally at 25-30 °C and pH 7.0-7.5 in the presence of 3 % (w/v) sea salts. The major cellular fatty acids were C_16 : 1ω6c and/or C_16 : 1ω7c, C_18 : 1ω6c and/or C_18 : 1ω7c, and C_16 : 0. The major isoprenoid quinone was Q-8. The genomic DNA G+C content was 56.1-57.2 mol%. Based on the phylogenetic, chemotaxonomic, genomic and phenotypic data presented, a novel species with the name Marinobacterium profundum sp. nov. is proposed, with PAMC 27536^T ( = KCCM 43095^T = JCM 30410^T) as the type strain.