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Updated: Mar 26, 2026

Proteome-wide Quantification of Labeling Homogeneity at the Single Molecule Level
Published on: April 19, 2019
Single-molecule Spectroscopy: Exploring Heterogeneity in Chemical and Biological Systems
Shirsendu Ghosh1, Kankan Bhattacharyya1
1Department of Physical Chemistry, Indian Association for the Cultivation of Science, 2A and 2B, Raja Subodh Chandra Mullick Rd, Jadavpur, Kolkata, West Bengal, 700032, India.
Abstract:
Many chemical and biological systems are heterogeneous in the molecular length scale (∼ 1 nm). Heterogeneity in many chemical systems and organized assemblies may be monitored using single-molecule spectroscopy (SMS). In SMS, the size of the focal spot (i.e., the smallest region to be probed) is nearly half of the excitation wavelength (λ/2, i.e., 200-375 nm) for visible light (400-750 nm). We discuss how one can get spatial resolutions better than 200 nm using molecules as nanometric probes. We show that polymer hydrogels, lipid vesicles, room temperature ionic liquids (RTILs), and binary liquid mixtures exhibit such heterogeneity. Another important observation is solute-dependent friction in RTILs. In an RTIL, diffusion of an ionic solute is slower than that of a neutral solute.
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