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A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics
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High-throughput cell quantification assays for use in cell purification development - enabling technologies for cell

Sarah Zimmermann1, Sarah Gretzinger1, Christian Scheeder1

  • 1Karlsruhe Institute of Technology (KIT), Institute of Process Engineering in Life Science, Section IV: Biomolecular Separation Engineering (MAB), Karlsruhe, Germany.

Biotechnology Journal
|January 28, 2016
PubMed
Summary

High-throughput screening (HTS) requires accurate cell quantification. CellTiter-Glo™ and flow cytometry excel in HTS for cell-based products, offering broad ranges and buffer compatibility.

Keywords:
Cell quantification assaysCell-based productsDownstream processingHigh-throughput screening (HTS)

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Area of Science:

  • Biotechnology
  • Process Development
  • Cell-based Assays

Background:

  • High-throughput screening (HTS) is crucial for cell-based product development.
  • Robust cell quantification methods are essential for HTS technology advancement.

Purpose of the Study:

  • To compare state-of-the-art cell quantification methods for HTS platforms.
  • To evaluate method applicability in downstream processing of cell-based products.

Main Methods:

  • Evaluated sensitivity, dynamic range, and precision of four cell quantification methods.
  • Assessed method performance across various buffer compositions, medium densities, and viscosities.

Main Results:

  • CellTiter-Glo™ and flow cytometry demonstrated excellent high-throughput cell quantification capabilities.
  • Both methods exhibited broad working ranges (3-4 log) and robustness to buffer variations.
  • CyQuant® Direct and CellTracker™ showed narrower ranges and higher sensitivity to buffer changes.

Conclusions:

  • CellTiter-Glo™ is optimal for fast, sensitive quantification of single cell types.
  • Flow cytometry is the preferred method for complex cell mixtures in HTS.
  • This analysis aids in selecting appropriate methods for HTS in cell-based product downstream processing.