Related Experiment Video
Updated: Aug 19, 2026
An Effective Mouse Model of Unilateral Renal Ischemia-Reperfusion Injury
Published on: July 15, 2021
[Evaluate the early changes of renal ischemia-reperfusion by susceptibility weighted imaging:primary experiment
Jinggang Zhang1, Wei Xing2, Jie Chen1
1Department of Radiology, First People's Hospital of Changzhou, Changzhou 213003, China.
Objective:
To explore whether susceptibility weighted imaging (SWI) could diagnose the early changes of renal IRI in vivo by modeling the renal ischemia-reperfusion (IRI) of rabbit.
Methods:
Thrity four New Zealand white rabbits were chosen, the vesseles of the left kidney of rabbits were ligated for 60 minutes, then the renal ischemia-reperfusion model was established. Respectively, the rabbits before ligation, 0.5, 24 and 48 h after the ligation, were examined with MR, then the signal of the cortex and medulla was analyzed in SWI and T2WI, the imaging features were compared with histopathology.
Results:
The inner medullary of renal showed high signal, low signal in the outer medulla and mildly high in the cortex before ligation in T2WI sequence, which showed the typical three-band signal distribution, while the outer medulla showed wildly hypo-signal in SWI, the remnants showed high signal; kidney swelled significantly 0.5 hour after ligation, the stripe of cortex became thin, however, the inner medulla and outer medulla widened. The hypo-signal of the outer medulla faded, while the signal of inner medullary and cortical slightly elevated. The signal intensity of outer medulla lowered more obviously in SWI, the margin of the cortex and medulla became more clearly 24 and 48 h after ligation,However the signal of the outer medulla was higher than normal kidney in the SWI, and punctuate or stripe haemorrhage occurred in the outer medulla. Respectively, the number of punctuate, stripe haemorrhage detected by SWI in the out medulla of kidney 0.5, 24 and 48 h after ligation were 0, 2.50, 6.75 (F=52.17, P<0.01), Meanwhile, the number detected by T2WI was 0, 1.13, 2.88 (F=15.65, P<0.01); the number of hemorrhage which was detected by SWI and T2WI in the outer medulla among different timepoint have the statistical significance (t=4.83, P<0.01).
Conclusion:
SWI can clearly show early changes in IRI-renal, especially in the outer medulla of the renal, the technique was helpful to assess the early changes of renal IRI.
