The isolation of B lymphocytes from human peripheral blood using antibodies coupled to paramagnetic particles and...

A method is described for the complete removal of monocytes and a reduction of natural killer cells from a suspension of peripheral blood mononuclear leukocytes. The cells are depleted in a batch procedure employing monoclonal antibodies coupled to paramagnetic particles. The bound cells are removed with the help of a magnet. The resulting cell population, which contains less than 1% esterase positive cells and a fraction of the natural killer cells originally present, can be further depleted of T lymphocytes by erythrocyte-rosetting techniques.