Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Quantitative antibody ELISA for leprosy.

R Hasan1, H M Dockrell, T Chiang

  • 1Department of Microbiology, Aga Khan University, Karachi Pakistan.

International Journal of Leprosy and Other Mycobacterial Diseases : Official Organ of the International Leprosy Association
|December 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A posttranslational proteomic survey of a single anatomically preserved human 20-week postconception brain.

Journal of anatomy·2026
Same author

CLINICAL AND MICROBIOLOGICAL ASSESSMENT OF CHLORHEXIDINE IMPACT ON GINGIVAL TISSUE RESPONSE AND BIOFILM FORMATION RELATED TO MATERIAL COMPOSITION IN FIXED PROSTHODONTIC RESTORATIONS.

Georgian medical news·2026
Same author

Ligand-receptor interactions of V-domain Ig-containing suppressor of T cell activation and programmed death-1 suppress the anticancer activities of T cells.

Immuno-oncology technology·2026
Same author

Search for Extremely-High-Energy Neutrinos and First Constraints on the Ultrahigh-Energy Cosmic-Ray Proton Fraction with IceCube.

Physical review letters·2025
Same author

Impact of helminth co-infection and treatment on mycobacterial growth inhibition in UK migrants with TB infection.

IJTLD open·2025
Same author

Measurement of Atmospheric Neutrino Oscillation Parameters Using Convolutional Neural Networks with 9.3 Years of Data in IceCube DeepCore.

Physical review letters·2025

Quantitative enzyme-linked immunosorbent assays (ELISAs) were developed to measure antibodies against Mycobacterium leprae in leprosy patients. Lepromatous leprosy showed significantly higher IgM levels compared to tuberculoid leprosy.

Area of Science:

  • Immunology
  • Microbiology
  • Medical Diagnostics

Background:

  • Leprosy, a chronic infectious disease caused by Mycobacterium leprae, exhibits diverse clinical manifestations.
  • Accurate serological markers are crucial for diagnosing and understanding leprosy pathogenesis.
  • Previous studies have explored various Mycobacterium leprae antigens for antibody detection.

Purpose of the Study:

  • To establish and validate quantitative enzyme-linked immunosorbent assays (ELISAs) for measuring IgM and IgG antibodies against specific Mycobacterium leprae antigens.
  • To compare antibody levels in different leprosy patient groups (lepromatous vs. tuberculoid) and controls.

Main Methods:

  • Development of quantitative ELISAs using soluble Mycobacterium leprae sonicate and a synthetic disaccharide antigen.

Related Experiment Videos

  • Inclusion of 46 leprosy patients and establishment of reference pools for IgM and IgG antibodies.
  • Expression of antibody titers in arbitrary units calibrated against reference pools.

    • Main Results:

    • The developed ELISAs demonstrated high specificity and reproducibility for both IgM and IgG antibody detection.
    • Lepromatous leprosy patients exhibited 10- to 100-fold higher IgM levels compared to controls.
    • Tuberculoid leprosy patients showed very low to undetectable IgM levels, while IgG levels showed overlap between groups.

    Conclusions:

    • Optimized ELISAs provide a reliable tool for quantifying IgM and IgG antibodies to Mycobacterium leprae antigens.
    • The distinct IgM profiles observed in lepromatous versus tuberculoid leprosy support their utility in disease classification.
    • These assays can facilitate future research on antibody dynamics during treatment and disease progression.