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Updated: Mar 26, 2026
Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
Published on: January 12, 2015
Surface proteins and osteoblast markers: characterization of human adipose tissue-derived osteogenic cells
O A Trentz1,2, D Arikketh3, V Sentilnathan3
1MIR (MIOT Institute of Research), MIOT Hospitals, Chennai, India. omanach@yahoo.com.
Introduction:
In adipose tissue-derived osteogenic cells (ADOC), osteoblast markers and surface proteins were determined and compared with osteoblasts harvested from cancellous bone (OB).
Method:
Osteocalcin (OC), core binding factor 1 (CBFA1), collagen type 1 (Coll1), alkaline phosphatase (ALP), nucleostemin (NS), and surface proteins CD 10, CD44, CD 59 and CD 105 were analyzed using RT-PCR, immunofluorescence and Western blot.
Results:
Osteocalcin expression was more distinct in OB than in ADOC, but the other markers and surface proteins showed no differences.
Conclusion:
These data support the use of adipose tissue for future regenerative medicine; however, further studies are necessary to establish the role of long-term differentiation.
