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Partial denture metal framework may harbor potentially pathogenic bacteria.

Cristiane Machado Mengatto1, Leonardo Marchini2, Luciano Angelo de Souza Bernardes3

  • 1Department of Conservative Dentistry, Federal University of Rio Grande do Sul, School of Dentistry, Porto Alegre, Rio Grande do Sul, Brazil.

The Journal of Advanced Prosthodontics
|January 28, 2016
PubMed
Summary

Bacterial diversity on removable partial denture (RPD) clasps changes over time, with potentially pathogenic bacteria increasing after four months. Regular dental visits and oral hygiene are recommended for RPD wearers.

Keywords:
16S rDNA geneDenture plaqueFrameworkMicroorganismsRemovable partial denture

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Area of Science:

  • Oral microbiology
  • Prosthodontics
  • Molecular biology

Background:

  • Removable partial dentures (RPDs) are common prosthetic devices.
  • The microbial biofilm on RPDs can impact oral health.
  • Understanding biofilm dynamics on RPD frameworks is crucial.

Purpose of the Study:

  • To characterize and compare bacterial diversity on removable partial denture (RPD) frameworks over time.
  • To identify changes in microbial communities on RPD clasps after insertion.

Main Methods:

  • A pilot study involved five women fitted with mandibular RPDs.
  • Biofilm samples from T-bar clasps were collected at 1 week (t1) and 4 months (t2) post-insertion.
  • Bacterial 16S rDNA was analyzed using PCR, cloning, and sequencing, with comparisons to GenBank.

Main Results:

  • Phylogenetic analysis revealed shifts in bacterial communities between t1 and t2.
  • Firmicutes dominated at t1 (82%), decreasing to 60% at t2, while other phyla like Proteobacteria and Fusobacteria increased.
  • Potentially pathogenic bacteria, including *F. nucleatum*, became more prevalent at t2.

Conclusions:

  • The biofilm composition on RPD metal clasps evolves with wear time.
  • RPD frameworks can harbor potentially pathogenic bacteria.
  • Regular follow-up and patient education on prosthesis care are advised.