Related Experiment Video

Updated: Mar 26, 2026

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

10.8K

First-trimester multimarker prediction of gestational diabetes mellitus using targeted mass spectrometry

Tina Ravnsborg1,2, Lise Lotte T Andersen3, Natacha D Trabjerg4

  • 1Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, Sdr. Boulevard 29, 5000, Odense C, Denmark.

Diabetologia
|January 29, 2016
PubMed

Insights

Predicting gestational diabetes mellitus (GDM) early is crucial. Multimarker panels of serum proteins show promise for improving first-trimester GDM prediction in pregnant women.

Area of Science:

  • Biochemistry
  • Clinical Chemistry
  • Reproductive Medicine

Background:

  • Gestational diabetes mellitus (GDM) poses risks like pre-eclampsia and future type 2 diabetes for mother and child.
  • Accurate early GDM prediction is vital for timely intervention and improved perinatal outcomes.
  • Current single protein biomarkers lack sufficient predictive power for GDM.

Purpose of the Study:

  • To investigate if multimarker panels of serum proteins enhance first-trimester GDM prediction.
  • To compare the predictive performance of single markers versus multimarker panels in obese and non-obese women.
  • To identify potential protein biomarkers for early GDM detection.

Main Methods:

  • A nested case-control study utilized first-trimester serum samples from GDM cases and controls.
  • Serum protein analysis was performed using targeted nano-flow liquid chromatography (LC) MS.
  • A 25-plex multiple reaction monitoring (MRM) MS assay was developed and validated.

Main Results:

  • Six proteins, including adiponectin, apolipoprotein M, and apolipoprotein D, were significantly different between obese GDM patients and controls.
  • Multimarker models combining protein levels and clinical data demonstrated improved predictive accuracy (AUC) compared to adiponectin alone.
  • These models showed enhanced prediction for obese, non-obese, and overall GDM groups.

Conclusions:

  • Multimarker models integrating protein markers and clinical data hold potential for predicting high-risk GDM pregnancies.
  • This approach may facilitate earlier identification of women susceptible to GDM.
  • Further validation is needed to establish these panels for routine clinical use.
Abstract

Keywords:
AdipokineApolipoproteinEarly predictionGestational diabetes mellitusObesityTargeted MS

More Related Videos

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure09:28

One-step Metabolomics: Carbohydrates, Organic and Amino Acids Quantified in a Single Procedure

Published on: June 25, 2010

13.7K
A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases06:50

A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases

Published on: May 29, 2020

3.2K

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.8K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
7.5K
Diabetes Mellitus: Type 2 and Gestational01:22

Diabetes Mellitus: Type 2 and Gestational

Type 2 diabetes, characterized by insulin resistance, arises when the insulin receptors on cells lose responsiveness to insulin, diminishing the cell's capacity to take up glucose, resulting in elevated blood glucose levels. To receive a diagnosis of Type 2 diabetes, a series of blood glucose tests are necessary to assess whether the blood glucose falls within normal parameters. If the result is out of the normal range, a patient may be diagnosed as prediabetic or diabetic, depending on the...
5.5K