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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
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IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration01:16

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A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
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IR Spectroscopy: Molecular Vibration Overview01:24

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When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
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Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy
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Label-Free Fluctuation Spectroscopy Based on Coherent Anti-Stokes Raman Scattering from Bulk Water Molecules.

M D Rabasovic1,2, E Sisamakis1,3, S Wennmalm4

  • 1Dept. Exp. Biomolecular Physics/Applied Physics, Royal Institute of Technology-KTH, Albanova University Center, 10691, Stockholm, Sweden.

Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry
|January 29, 2016
PubMed
Summary
This summary is machine-generated.

A new label-free method, inverse CARS-based correlation spectroscopy (iCARS-CS), analyzes nanoparticles and vesicles. This technique uses water

Keywords:
CARS (coherent anti-Stokes Raman scattering)FCS (fluorescence correlationspectroscopy)label-freemicroparticlesnanoparticles

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Nanotechnology

Background:

  • Inverse fluorescence correlation spectroscopy (iFCS) analyzes particles but requires high fluorophore concentrations.
  • Label-free analysis of nanoparticles (NPs) and molecules is challenging.
  • Existing methods often necessitate labeling of the analytes.

Purpose of the Study:

  • To develop a fully label-free method for analyzing nanoparticles and vesicles.
  • To overcome the limitations of high fluorophore concentrations in iFCS.
  • To enable sensitive detection and quantification of small particles in solution.

Main Methods:

  • Utilized coherent anti-Stokes Raman scattering (CARS) from water molecules as the signal source.
  • Developed inverse CARS-based correlation spectroscopy (iCARS-CS).
  • Analyzed nanoparticles and lipid vesicles as they passed through a detection volume.

Main Results:

  • Successfully analyzed nanoparticles (few tenths of nm diameter) at picomolar concentrations.
  • Enabled determination of absolute volumes and concentrations of nanoparticles.
  • Demonstrated analysis of lipid vesicles using CARS fluctuations from surrounding water.

Conclusions:

  • iCARS-CS offers a broadly applicable, label-free characterization technique.
  • The method is suitable for analyzing nanoparticles, exosomes, and microparticles.
  • Potential applications in biomolecular diagnostics and screening are highlighted.