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Updated: Sep 12, 2026
Phenotypic Characterization of Macrophages from Rat Kidney by Flow Cytometry
Published on: October 18, 2016
Peritoneal dialysis fluid induces change of mononuclear phagocyte proportions
H J Bos1, F Meyer, J C de Veld
1Department of Cell Biology, Faculty of Medicine, Free University, Amsterdam, The Netherlands.
Abstract:
Peritoneal macrophages from uninfected continuous ambulatory peritoneal dialysis (CAPD) patients in general show two different, endogenous peroxidase activity (PA) patterns: exudate and negative. This suggests, in accordance with the animal model, that these macrophages are changed proportionately in CAPD patients. This chronic change may be caused by mechanical stimulation alone (massage) or the composition of the dialysis fluid used. Therefore in the rat model both physiological saline and commercial dialysis fluid were intraperitoneally (i.p.) administrated. Our results on the PA-pattern of peritoneal macrophages do indicate that a single i.p. administration of commercial dialysis fluid induced an acute exudate, especially when compared with the minor saline effect. These results are confirmed by the percentage of macrophages positive for a differentiation antigen recognized by the monoclonal antibody ED2. In addition the percentage of Fc-receptor positive peritoneal cells is more enhanced after i.p. injection of dialysis fluid when compared with the saline effect. These findings strongly suggest that the dialysis fluid used in peritoneal dialysis patients is the inducer of exudate peritoneal macrophages in these patients.
Insights
Dialysis fluid, not mechanical stimulation, induces exudate peritoneal macrophages in continuous ambulatory peritoneal dialysis (CAPD) patients. This finding highlights the impact of dialysis fluid composition on macrophage activity during CAPD.
Area of Science:
- Immunology
- Nephrology
Background:
- Peritoneal macrophages in continuous ambulatory peritoneal dialysis (CAPD) patients exhibit distinct peroxidase activity (PA) patterns.
- These changes suggest macrophage alterations in CAPD patients, potentially due to mechanical stimulation or dialysis fluid composition.
Purpose of the Study:
- To investigate the role of dialysis fluid versus mechanical stimulation in inducing changes in peritoneal macrophages.
- To determine the primary inducer of exudate peritoneal macrophages in CAPD patients.
Main Methods:
- Administered physiological saline and commercial dialysis fluid intraperitoneally (i.p.) in a rat model.
- Analyzed peritoneal macrophage peroxidase activity (PA) patterns.
- Quantified macrophages expressing the differentiation antigen ED2 and Fc-receptors.
Main Results:
- Commercial dialysis fluid i.p. administration induced acute exudate peritoneal macrophages, unlike physiological saline.
- Dialysis fluid increased the percentage of ED2-positive macrophages compared to saline.
- Dialysis fluid enhanced Fc-receptor positive peritoneal cells more than saline.
Conclusions:
- Dialysis fluid, not mechanical factors alone, is the primary inducer of exudate peritoneal macrophages in CAPD patients.
- These findings emphasize the immunomodulatory effects of dialysis fluid on the peritoneal environment.
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