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Related Experiment Video

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Surface-modified complex SU-8 microstructures for indirect optical manipulation of single cells.

Badri L Aekbote1, Tamás Fekete1, Jaroslaw Jacak2

  • 1Biological Research Centre of the Hungarian Academy of Sciences, Temesvári krt. 62, Szeged 6726, Hungary.

Biomedical Optics Express
|January 29, 2016
PubMed
Summary

This study presents a novel method for manipulating live cells using 3D microstructures created with two-photon polymerization (TPP) and surface functionalization. This technique allows for precise, indirect optical control of cells while maintaining a safe distance for sensitive biological applications.

Keywords:
(140.7090) Ultrafast lasers(160.1435) Biomaterials(160.5470) Polymers(180.2520) Fluorescence microscopy(230.4000) Microstructure fabrication(230.6120) Spatial light modulators(310.0310) Thin films(350.3390) Laser materials processing(350.4855) Optical tweezers or optical manipulation

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Area of Science:

  • Biotechnology
  • Optical Engineering
  • Cell Biology

Background:

  • Optical manipulation of live cells is crucial for various biological studies.
  • Existing methods may pose risks to sensitive cells due to proximity.
  • Advanced microfabrication and surface chemistry are needed for improved cell handling.

Purpose of the Study:

  • To develop a method for indirect optical manipulation of live cells.
  • To create 3D microstructures capable of specific cell attachment.
  • To enable precise 6-degrees-of-freedom actuation of cells using optical forces.

Main Methods:

  • Combining two-photon polymerization (TPP) for 3D microstructure fabrication.
  • Surface functionalization of TPP structures with streptavidin and IgG antibodies via polyethylene glycol diamine linker.
  • Quantification of protein density on microstructures.
  • Utilizing multi-focus optical traps for indirect cell manipulation.

Main Results:

  • Successfully fabricated TPP microstructures with specific streptavidin and non-specific IgG antibody coatings.
  • Demonstrated reproducible attachment of streptavidin-coated structures to biotinylated cells.
  • Performed indirect optical micromanipulation tasks on attached structure-cell couples.
  • Achieved 6-degrees-of-freedom actuation of cells using extended manipulators.

Conclusions:

  • The combined TPP and surface functionalization method enables effective indirect optical manipulation of live cells.
  • This approach allows for safe manipulation by maintaining distance between trapping beams and cells.
  • The developed technique offers precise control and actuation for advanced cell-based research.