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Effective Sample Preparations in Imaging Mass Spectrometry.

Shuichi Shimma1, Yuki Sugiura2

  • 1Division of Translational Research, Exploratory Oncology Research & Clinical Trial Center, National Cancer Center; Division of Clinical Pharmacology Group for Translational Research Support Core, National Cancer Center Research Institute.

Mass Spectrometry (Tokyo, Japan)
|January 29, 2016
PubMed
Summary

Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) visualizes biomolecules on tissues. Proper sample preparation, including matrix solvent choice, is critical for high-sensitivity MALDI-IMS results.

Keywords:
adenylatesanti-cancer agentsfocused-microwave irradiationimaging mass spectrometrysample preparation

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry

Background:

  • Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) is a powerful technique for spatially mapping biomolecules and drugs within tissue sections.
  • Successful MALDI-IMS analysis relies heavily on meticulous sample preparation, as various factors can significantly influence data quality and interpretation.
  • Understanding the impact of each preparation step is essential for optimizing experimental outcomes.

Purpose of the Study:

  • To provide a comprehensive review of sample preparation techniques for MALDI-IMS.
  • To detail each step of the sample preparation workflow with practical examples.
  • To emphasize the critical role of the organic solvent composition in matrix solutions for enhancing sensitivity.

Main Methods:

  • Review of existing literature and best practices in MALDI-IMS sample preparation.
  • Systematic organization of sample preparation steps, from tissue sampling to matrix application.
  • Discussion on the influence of key parameters, including tissue handling, thickness, and matrix application methods.
  • Analysis of the impact of organic solvent composition in matrix solutions.

Main Results:

  • Sample preparation significantly impacts MALDI-IMS data quality, affecting the visualization of biomolecules and drugs.
  • Tissue sampling methods, thickness, and matrix application procedures are critical variables that require careful control.
  • The choice and composition of organic solvents within the matrix solution are paramount for achieving high sensitivity in MALDI-IMS.

Conclusions:

  • Optimized sample preparation is indispensable for reliable and sensitive MALDI-IMS analysis.
  • A systematic approach to sample preparation, considering all critical steps, is necessary for successful biomolecule and drug distribution mapping.
  • Further research into matrix solution optimization, particularly organic solvent composition, can lead to improved MALDI-IMS performance.