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Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
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Higher urine heat shock protein 70/creatinine ratio in type 1 diabetes mellitus

Alev Yilmaz1, Asuman Gedikbasi2, Zeynep Yuruk Yildirim1

  • 1a Pediatric Nephrology Department, Istanbul Medical Faculty , Istanbul University , Istanbul , Turkey ;

Renal Failure
|January 29, 2016
PubMed

Insights

Urine heat shock proteins (HSPs) are elevated in children with type 1 diabetes mellitus (T1DM), indicating progressive kidney damage. Urine heat shock protein 70 (uHSP70/Cr) levels increase over time, serving as a potential early biomarker for diabetic nephropathy.

Area of Science:

  • Nephrology
  • Endocrinology
  • Biochemistry

Background:

  • Type 1 diabetes mellitus (T1DM) can cause insidious progressive renal damage starting in childhood.
  • Heat shock proteins (HSPs) are crucial in cellular responses to stress and damage.
  • Early detection of renal injury in diabetic children is vital for timely intervention.

Purpose of the Study:

  • To investigate if urine HSP levels increase in diabetic children over time.
  • To determine if elevated urine HSPs indicate progressive renal injury in T1DM.
  • To assess the potential of urine HSPs as early biomarkers for diabetic nephropathy.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA) was used to measure urine HSP27, HSP40, HSP60, HSP70, and HSP90 levels.
  • Measurements were taken from 33 T1DM patients and 24 healthy children over two consecutive years (2012 and 2013).
  • Results were normalized to creatinine levels (uHSP/Cr) and analyzed for significant changes and predictive value.

Main Results:

  • Urine HSP levels (HSP27/Cr, HSP40/Cr, HSP60/Cr, HSP70/Cr, HSP90/Cr) were significantly higher in diabetic children compared to controls in 2012.
  • In 2013, urine HSP70/Cr levels significantly increased, while HSP60/Cr decreased, and others remained stable.
  • Urine HSP70/Cr demonstrated high diagnostic accuracy (AUC 0.957) with 85% sensitivity and 96% specificity for predicting diabetic kidney damage.

Conclusions:

  • Elevated urine HSP levels are present in children with T1DM, suggesting early renal stress.
  • Increasing urine HSP70/Cr levels over time may signify progressive kidney damage in T1DM.
  • Urine HSP70/Cr shows promise as a sensitive and specific non-invasive biomarker for early detection of diabetic nephropathy in children.

Keywords:
Childrendiabetesdiabetic nephropathyheat shock proteinnephropathy

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