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Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System.
James A Fornwald1, Quinn Lu2, Frederick M Boyce3
1Platform Technology and Science, GlaxoSmithKline, 1250 S. Collegeville Road, Collegeville, PA, 19426, USA. Jim.A.Fornwald@gsk.com.
Methods in Molecular Biology (Clifton, N.J.)
|January 29, 2016
Summary
Modified baculoviruses, known as BacMams, offer an efficient method for gene delivery and expression in mammalian cells. These vectors are easily constructed and utilized with standard molecular biology techniques, simplifying research workflows.
Area of Science:
- Molecular Biology
- Virology
- Gene Therapy
Background:
- Baculoviruses are widely used for gene expression in insect cells.
- Mammalian gene expression systems require efficient and reliable delivery vectors.
- BacMams are engineered baculoviruses for mammalian gene delivery.
Purpose of the Study:
- To introduce BacMams as a valuable tool for mammalian gene expression.
- To describe the construction and generation of BacMam vectors.
- To highlight the ease of BacMam transduction in mammalian cells.
Main Methods:
- Construction of BacMam transfer vectors using standard molecular biology.
- Generation of BacMam virus particles utilizing established baculovirus methods.
- Transduction of mammalian cells with BacMam vectors with minimal protocol changes.
Main Results:
- BacMams facilitate gene delivery and expression in mammalian cells.
- Transfer vector construction is straightforward.
- Virus generation and cell transduction are based on common laboratory practices.
Conclusions:
- BacMams are an integral part of the recombinant mammalian gene expression toolbox.
- The use of BacMams simplifies gene delivery and expression in mammalian systems.
- The BacMam transfer vector pHTBV is a key component for these applications.
